A needle to protect life! Nannong Gaoke pig pseudorabies live vaccine (TP strain) was released

On June 24, the high-quality development conference and New product launch conference sponsored by Jiangsu Nannong High-tech Co., Ltd. was held in Hainan. Customers from all over the country, with their expectations for the future of animal husbandry, met in Boao, a vibrant land. We witnessed a milestone moment - the glorious listing of Nannong Hi-Tech pig Pseudorabies live vaccine (TP strain), which marked another major breakthrough in the field of animal disease prevention and control, indicating that the breeding industry is moving towards a healthier and safer direction.

Zhou Cheng, chairman of Jiangsu Nannong High-tech Co., LTD., delivered the opening speech for the conference

Zhou Cheng, chairman of Jiangsu Nannong High-tech Co., Ltd. expressed his warmest welcome and heartfelt thanks to the guests attending the conference. In his speech, Chairman Zhou reviewed the development history of Nannong High-tech, from a single product in the initial stage to now having 32 product production numbers; From a single product production line to now has 2 GMP production workshops 12 production lines; From a single product category to today's pig vaccine series, poultry vaccine series, sheep vaccine series and many other product systems; From the single whole virus rotating bottle production process to today's cutting-edge technology such as vaccine high density fermentation and genetic engineering suspension culture process, the company has been continuously upgraded in the industry ranking, and has also been identified as a national high-tech enterprise, a national specialized new "little giant" enterprise. As the flagship product of Nannong Hi-Tech pig pseudorabies vaccine series, live pig pseudorabies vaccine (TP strain) will surely become the third core product series star after the company's two core product series of Ring and blue ear, creating a new era of pig pseudorabies disease prevention and control, and helping the majority of users to raise pigs and increase their harvest!

5 new products: Swine pseudorabie live vaccine (TP strain), recombinant Newcastle disease virus, avian influenza virus (H9 subtype) two inactivated vaccine (A-ⅶ strain +WJ57 strain), chicken infectious rhinitis trivalent inactivated vaccine (A type HN3 strain +B type HN5 strain +C type SD3 strain), duck Tambusu virus disease live vaccine (FX2010-180P strain), duck infectious serositis, The inactivated colibacillosis vaccine (type 2 RA BYT06 strain +O78 EC BYT01 strain) was successfully released

Pig pseudorabies was first reported in 1813 and has since become widespread in European pig herds with the spread of intensive pig farming. Since the 1970s, China's pig farms have suffered large-scale outbreaks of pseudorabies. With the introduction of natural attenuated vaccine strain BarthaK-61 in 1979 and the development of the attenuated vaccine, the epidemic was controlled. After 1990, the epidemic situation decreased significantly, and most large-scale pig farms have basically achieved purification of pseudorabies. In this context, the state proposed to purify the disease in all breeding farms in the country by 2020.

However, at the end of 2011 and the beginning of 2012, many pig farms in North China (mainly Henan, Shandong, Hebei) that had been immunized with live vaccine of pig pseudorabies gene deletion still had suspected outbreaks of pig pseudorabies, with newborn piglets showing typical neurological symptoms and mortality approaching 100%. Mass abortion of pregnant sows.

Tian Zhijun, Professor of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, shared "The Discovery of Chinese Pig Pseudorabies Mutant Strain and Its Vaccine Development"

Researcher Tian Zhijun said that a large number of wild pseudorabies strains could be detected and isolated in pig farms with suspected pseudorabies and were immunized with live pseudorabies vaccine. By comparing the gE nucleotide sequences of the newly isolated PRVS, it was found that the PRVS isolated from 2012 to now are located in a relatively independent branch (genotype II variant), and have the same molecular characteristics, and are relatively distant from the previously isolated strains.

Professor Wang Jichun of Jiangsu Academy of Agricultural Sciences shared "Key Technologies for Prevention, Control and Purification of Pseudorabies Virus Mutation in Large-scale Pig Farms"

The emergence and outbreak of Pseudorabies virus genotype II variant have brought huge economic losses to China's pig breeding industry. So is the new strain more virulent? Are there quality problems with existing vaccines? How effective is the protection against new strains?

Tian Zhijun, a researcher, proposed that the LD50 test of mice after virus isolation found that the new PRV strain (HeN1) had a high fatality rate at a low age (within 2 months of age), indicating that the virulence of the variant strain was enhanced. Bartha K61 vaccine was used to perform the attack protection experiments respectively, and it was found that the attack protection rate against HeN1 strain was only 50%, and the attack protection against S strain was still 100%, indicating that the immunogenicity of the vaccine had not changed, but Bartha k61 vaccine could only provide partial protection against newly isolated porcine pseudorabies virulence (HeN1 strain).

At the same time, Professor Wang Jichun and his team also carried out a number of protection tests, showing that Bartha K61 vaccine can produce effective protection against the mutant strain, but it needs to strengthen the immunization several times; Vaccination can effectively produce protection, but it can not completely prevent infection and detoxification, and a strong infection can form a lifelong latent infection.

It can be seen that the existing porcine pseudorabies vaccine has obvious defects in the clinical protection and detoxification of the mutant strains!

Aiming at the above difficulties, Jiangsu Nannong Hi-tech Technology Co., Ltd. and Harbin Veterinary Research Institute jointly tackled the key problems and obtained the 5 gene deletion weak strain (TP strain) of porcine Pseudorabies virus variant. The vaccine "Huawei Qing" produced by TP strain can not only provide good clinical protection for pigs, but also effectively prevent the spread of the virus in pig farms, which plays an important role in the prevention and control and purification of pig pseudorabies in pig farms.

Compared with classic vaccines, "Huawei Qing" has the following characteristics:

1. The source strain was a mutant strain with high production and stable passage

After the new variant strain HeN1 was inoculated into suitable cells, the virus propagated rapidly from the fourth generation, and the _TCID50 of the fourth and fifth generation of the virus could reach ^108/0.1mL and be stable after inoculation with the ratio of 1:1000.

2. Vaccine strain 5 gene deletion, good safety

A vaccine strain with 5 gene deletions (including TK, g, gE, Us9, Us2 and some reverse repeats) was obtained by low temperature passage and drug screening. The vaccine produced by this strain has high safety, and all sheep and pigs are alive after immunization with _108TCID50 virulence, and there is no vertical and horizontal transmission. The virus was not isolated and nucleic acid was not detected in immunized pig tissue after 10 times dose immunization.

3. Good immunogenicity, can effectively prevent PRV variant strains from detoxifying in pigs

The piglets were challenged after immunization, and the immune group was completely protected, while the control group died at least 4/5. Anal swab results showed that the virus titer in the immune group was significantly lower than that in the immune group of Bartha-K61 strain at 4 days after the challenge, and no virus was detected at 7 days after the challenge, effectively preventing the spread of PRV variant strain in pigs.

4. The latest freeze-drying process for vaccine production ensures the stability of vaccine quality

The vaccine is prepared with new production technology and the latest freeze-drying equipment, and the quality of the vaccine is uniform and stable.

5. One immunization, protective antibodies maintained for more than 6 months

Antibodies were produced 7 days after immunization, and high levels of antibodies were maintained for more than 6 months.

Tian Zhijun, a researcher, said that the live porcine pseudorabies vaccine (TP strain) was safe and effective. Sow 2 times a year, piglet "a needle out", the smallest number of immunizations can achieve the best immune effect, reducing the burden for the pig farm; In addition, the probability of re-infection of pig pseudorabies virus in pig farms is low, and the negative time is long, which is the best product for prevention and control and purification of pig pseudorabies variant strains.

In order to celebrate the successful launch of new products, Nannong High-tech carefully prepared a high-tech drone performance. When night fell, dozens of drones took off in formation, changing the formation in the air with bright lights, not only showing the name and logo of the vaccine product, but also vividly representing the important significance of the vaccine for the prevention and control of pseudorabies in pigs, bringing a visual feast for the guests and the audience, but also symbolizing the latest achievements and bright prospects of scientific and technological innovation in the field of animal protection.